6.25pm: Earlier ‘leaked’ team news suggested that England boss Gareth Southgate will stick with Phil Foden and Raheem Sterling either side of goalless frontman Harry Kane.
But it’s thought Jack Grealish is also primed to start while Harry Maguire could displace Tyrone Mings at centre-back.
Jadon Sancho will start on the substitutes’ bench having yet to play a single minute of the tournament until now.
Chilwell has yet to feature at the Euros and Southgate has Luke Shaw, Kieran Trippier and even Bukayo Saka as options in that position.
The full confirmed line-ups are expected shortly – and we’ll see how many changes Southgate makes from the Scotland draw.
6.15pm: England will be without Mason Mount and Ben Chilwell this evening with the Chelsea pair in isolation.
The duo were in close contact with Scotland’s Billy Gilmour after Friday night’s game with the midfielder since testing positive for coronavirus.
They will isolate for 10 days, meaning they might also be unavailable for next week’s last 16 tie.
The FA confirmed on Tuesday morning: “Chilwell and Mount will isolate and train individually in private areas at England’s training base St George’s Park, with the rest of the squad returning there after tonight’s fixture against Czech Republic at Wembley.
“We will continue to follow all Covid-19 protocols and the UEFA testing regime, while remaining in close contact with PHE.
“The entire England squad and staff had lateral flow tests on Monday and all were again negative, as was the case with Sunday’s UEFA pre-match PCR tests. Further tests will be carried out as and when appropriate.”
6pm: Good evening and welcome to Express Sport’s live coverage of England’s final Euro 2020 Group D match against Czech Republic at Wembley.
Having beaten Croatia but drawn with Scotland, Gareth Southgate’s side are already into the knockout stages of the Euros.
The four best third-placed teams from the six groups make it through to the last 16 and Ukraine and Finland finished third in their groups on Monday night on three points apiece.
That means even if England finish third tonight, which they will if they lose and Scotland win, they will still progress.
Tonight’s opponents Czech Republic beat Scotland and drew with Croatia but are above England thanks to their superior goal difference.